- coli containing pKD46 and pEH-1-YidC-GFP-Urbanus were obtained as colonies on LB agar. pCas9 and pCRISPR were obtained as E. colistab cultures (Addgene). Plasmid DNA was isolated from overnight cultures using the Purelink Quick Plasmid Miniprep Kit (ThermoFisher) according to the manufacturer's instructions.
- The study presents a CRISPR/Cas9‐mediated genomic error‐prone editing (CREPE) technology for generating a library of mutants within a targeted Escherichia coli gene in its native genomic context.
- Apr 30, 2021 · They inserted these retron plasmids into E. coli bacteria to see if the genes were successfully integrated into their genomes after 20 generations of cell replication. Initially, less than 0.1% of E. coli bearing the retron recombineering system incorporated the desired mutation.
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